Human CD34 antibody

• Description:

  CD34, also known as hematopoietic progenitor cell antigen CD34 isoform b, is a member of a family of single-pass transmembrane sialomucin proteins. It is adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. This protein could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.

• Product Name Alternative:

  Hematopoietic progenitor cell antigen CD34 isoform b, CD34

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human CD34 (32-290aa) purified from Baculovirus.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  AT3B6

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, FACS

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  CD34, Hematopoietic progenitor cell antigen CD34 isoform b, CD34, ATGA0497-10 µg, ATGA0497-20 µg, ATGA0497-50 µg, ATGA0497-100 µg, ATGA0497-250 µg, ATGA0497-500 µg, ATGA0497-1 mg, ATGA0497-10, ATGA0497-20, ATGA0497-50, ATGA0497-100, ATGA0497-250, ATGA0497-500, ATGA0497-1

• References & Citations:

  Nielsen JS., et al. (2009) Microcirculation. 16: 487-496.; ; Mastrandrea F., et al. (2008) Eur Ann Allergy Clin Immunol. 40: 90-103.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Neuroscience

• NCBI Accession Number:

  NP_001764

• Uniprot Accession Number:

  P28906

• WB Description:

  The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD34 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate

• FACS Description:

  Flow cytometry analysis of CD34 in KG1 cells. The cell was stained with ATGA0497 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .