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Human Integrin beta 1/CD29 antibody

CAT: 0112-ATGA0485-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0485-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Product Name Alternative
Integrin beta 1, Integrin beta 1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit bet
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ITGB1 (21-461aa) purified from E. coli.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT47E2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0485-10 µg, ATGA0485-20 µg, ATGA0485-50 µg, ATGA0485-100 µg, ATGA0485-250 µg, ATGA0485-500 µg, ATGA0485-1 mg, ATGA0485-010, ATGA0485-020, ATGA0485-050, ATGA0485-100, ATGA0485-250, ATGA0485-500, ATGA0485-01M
References & Citations
Pellinen T., et al. (2008) Dev. Cell. 15: 371-385.; ; Chuang N.N., et al. (2007) Biochem. Soc. Trans. 35:1292-1294.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_002202
Uniprot Accession Number
P05556
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ITGB1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A549 cell lysate ; ; Lane 2.: PC3 cell lysate ; ; Lane 3.: HT1080 cell lysate
IF Description
ICC/IF analysis of ITGB1 in HeLa cells. The cell was stained with ATGA0485 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0485-IF1b.jpg}

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