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Human HSPA13 antibody

CAT: 0112-ATGA0477-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0477-100Size:100 µL
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Description
HSPA13, also known as STCH, is a member of the heat shock protein 70 family and is found associated with microsomes. Members of this protein family play a role in the processing of cytosolic and secretory proteins, as well as in the removal of denatured or incorrectly-folded proteins. HSPA13 has been found to interact with PLIC-1 and PLIC-2, proteins involved in the signaling connection between the membrane receptors for thrombospondin and the cytoskeleton.
Product Name Alternative
Heat shock protein 70kDa family member 13, STCH
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human HSPA13 (23-471aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT2F6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
HSPA13, Heat shock protein 70kDa family member 13, STCH, ATGA0477-10 µg, ATGA0477-20 µg, ATGA0477-50 µg, ATGA0477-100 µg, ATGA0477-250 µg, ATGA0477-500 µg, ATGA0477-1 mg, ATGA0477-10, ATGA0477-20, ATGA0477-50, ATGA0477-100, ATGA0477-250, ATGA0477-500, ATGA0477-1
References & Citations
Rual JF., et al. (2005) Nature. 437 (7062) : 1173-8.; ; Lim J., et al. (2006) Cell. 125 (4) : 801-14.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
AAH36370
Uniprot Accession Number
P48723
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSPA13 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: MCF7 cell lysate
IF Description
ICC/IF analysis of HSPA13 in Hep3B cells. The cell was stained with ATGA0477 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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