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Human CTGF/CCN2 antibody

CAT: 0112-ATGA0468-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0468-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein) . CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.
Product Name Alternative
Connective tissue growth factor, CCN2, NOV2, HCS24, IGFBP8
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CTGF (27-349 aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT18E7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CTGF, CCN2, Connective tissue growth factor, CCN2, NOV2, HCS24, IGFBP8, ATGA0468-10 µg, ATGA0468-20 µg, ATGA0468-50 µg, ATGA0468-100 µg, ATGA0468-250 µg, ATGA0468-500 µg, ATGA0468-1 mg, ATGA0468-10, ATGA0468-20, ATGA0468-50, ATGA0468-100, ATGA0468-250, ATGA0468-500, ATGA0468-1
References & Citations
Jun JI., et al. (2011) Nat Rev Drug Discov. 10 (12) : 945-63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cardiovascular
NCBI Accession Number
NP_001892
Uniprot Accession Number
P29279
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTGF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: U87MG cell lysate ; ; Lane 4.: A427 cell lysate
IF Description
ICC/IF analysis of CTGF in Hep3B cells. The cell was stained with ATGA0468 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {ATGA0468-IF1b.jpg}

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