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Human AK1 antibody

CAT: 0112-ATGA0447-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0447-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.
Product Name Alternative
Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human AK1 (1-194aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT7E9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
AK1, Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, ATGA0447-10 µg, ATGA0447-20 µg, ATGA0447-50 µg, ATGA0447-100 µg, ATGA0447-250 µg, ATGA0447-500 µg, ATGA0447-1 mg, ATGA0447-10, ATGA0447-20, ATGA0447-50, ATGA0447-100, ATGA0447-250, ATGA0447-500, ATGA0447-1
References & Citations
Terzic A., et al. (2007) J Biol Chem. 282 (43) : 31366-72.; ; Morelli A., et al. (2007) Curr Eye Res. 32 (3) : 249-57.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000467
Uniprot Accession Number
P00568
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: U87-MG cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: 293T cell lysate ; ; Lane 5.: HepG2 cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: MCF7cell lysate ; ; Lane 8.: SK-OV-3 cell lysate ; ; Lane 9.: PC3 cell lysate
IF Description
ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .{ATGA0447-IF1b.jpg}ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .{ATGA0447-IF2a.jpg}{ATGA0447-IF2b.jpg}ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0447-IF3a.jpg}{ATGA0447-IF3b.jpg}

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