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Human IMP3 antibody

CAT: 0112-ATGA0437-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0437-100Size:100 µL
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Description
IMP3 is the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the u3 snoRNP complex. The protein contains an S4 domain.
Product Name Alternative
U3 small nucleolar ribonucleoprotein protein IMP3, BRMS2, C15orf12, MRPS4
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human IMP3 (1-184aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT32E9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
IMP3, u3 small nucleolar ribonucleoprotein protein IMP3, BRMS2, C15orf12, MRPS4, ATGA0437-10 µg, ATGA0437-20 µg, ATGA0437-50 µg, ATGA0437-100 µg, ATGA0437-250 µg, ATGA0437-500 µg, ATGA0437-1 mg, ATGA0437-10, ATGA0437-20, ATGA0437-50, ATGA0437-100, ATGA0437-250, ATGA0437-500, ATGA0437-1
References & Citations
Li C., et al. (2007) Mod Pathol. 20 (2) : 242-7. ; ; Granneman S., et al. (2003) Nucleic Acids Res. 31 (7) : 1877-87.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_060755
Uniprot Accession Number
Q9NV31
WB Description
The Cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IMP3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : IMP3 Transfected 293T cell lysate
IF Description
ICC/IF analysis of IMP3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IMP3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of IMP3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IMP3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0437-IF2.jpg}

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