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Human ALDOC antibody

CAT: 0112-ATGA0397-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0397-100Size:100 µL
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Description
ALDOC, also known as aldolase C, is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1, 6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain.
Product Name Alternative
Aldolase C fructose-bisphosphate., Aldolase C, fructose-bisphosphate., ALDC
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ALDOC (1-364aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT2E11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ALDOC, ATGA0397-10 µg, ATGA0397-20 µg, ATGA0397-50 µg, ATGA0397-100 µg, ATGA0397-250 µg, ATGA0397-500 µg, ATGA0397-1 mg, ATGA0397-10, ATGA0397-20, ATGA0397-50, ATGA0397-100, ATGA0397-250, ATGA0397-500, ATGA0397-1
References & Citations
Wang CF., et al. (2007) Anim Genet. 38 (3) : 203-210.; ; Arakaki TL., et al. (2004) Protein Sci. 13 (12) : 3077-3084.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_005156
Uniprot Accession Number
P09972
WB Description
The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : PC3 cell lysate; ; Lane 2. : U87MG cell lysate ; ; Lane 3. : WiDr cell lysate ; ; Lane 4. : Jurkat cell lysate ; ; Lane 5. : Mouse brain tissue lysate
IF Description
ICC/IF analysis of ALDOC in U87MG cells. The cell was stained with ATGA0397 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ALDOC in Jurkat cells. The cell was stained with ATGA0397 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0397-IF2.jpg}

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