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Human GPI antibody

CAT: 0112-ATGA0390-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0390-050Size:50 µL
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Description
Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme. In the cytoplasm, it functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P) . Extracellularly, it functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. GPI is also referred to as autocrine motility factor (AMF) based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in GPI are the cause of nonspherocytic hemolytic anemia, and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
Product Name Alternative
Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, EC 5.3.1.9, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36, G6PI, Glucose-6-phosphate isomerase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human GPI (1-558aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT22G2
Conjugation
Unconjugated
Applications
ELISA, WB, FACS, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
GPI, Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, EC 5.3.1.9, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, SA 36, Sperm antigen 36, G6PI, Glucose-6-phosphate isomerase, ATGA0390-10 µg, ATGA0390-20 µg, ATGA0390-50 µg, ATGA0390-100 µg, ATGA0390-250 µg, ATGA0390-500 µg, ATGA0390-1 mg, ATGA0390-10, ATGA0390-20, ATGA0390-50, ATGA0390-100, ATGA0390-250, ATGA0390-500, ATGA0390-1
References & Citations
Cordeiro AT., et al. (2003) Biochimica et biophysica acta. 1645 (2) : 117-122.; ; Kugler W., et al. (2000) Baillieres Best Pract Res Clin Haematol. 13 (1) : 89-101.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_000166
Uniprot Accession Number
P06744
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPI antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : HepG2 cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : Jurkat cell lysate ; ; Lane 5. : U87MG cell lysate ; ; Lane 6. : Raji cell lysate ; ; Lane 7. : MCF7 cell lysate
FACS Description
Flow cytometry analysis of GPI in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of GPI in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPI antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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