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Human PGK1 antibody

CAT: 0112-ATGA0381-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0381-100Size:100 µL
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Description
Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.
Product Name Alternative
Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PGK1 (1-417aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT2F4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PGK1, Phosphoglycerate kinase 1, Pgk-1, ATGA0381-10 µg, ATGA0381-20 µg, ATGA0381-50 µg, ATGA0381-100 µg, ATGA0381-250 µg, ATGA0381-500 µg, ATGA0381-1 mg, ATGA0381-10, ATGA0381-20, ATGA0381-50, ATGA0381-100, ATGA0381-250, ATGA0381-500, ATGA0381-1
References & Citations
Svaasand EK., et al. (2007) Muscle Nerve. 36 (5) : 679-684. ; ; Valentin C., et al. (1998) Hum Mutat. 12 (4) : 280-287.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000282
Uniprot Accession Number
P00558
WB Description
Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : LNCaP cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : 293T cell lysate ; ; Lane 6. : K562 cell lysate ; ; Lane 7. : Jurkat cell lysate
IF Description
ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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