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Human CAPNS1 antibody

CAT: 0112-ATGA0367-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0367-100Size:100 µL
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Description
CAPNS1, also known as Calpain small subunit 1, are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Two transcript variants encoding the same protein have been identified for this gene.
Product Name Alternative
Calpain small subunit 1, Calpain, small subunit 1, 30K, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CAPNS1 (84-268aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1D11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CAPNS1, ATGA0367-10 µg, ATGA0367-20 µg, ATGA0367-50 µg, ATGA0367-100 µg, ATGA0367-250 µg, ATGA0367-500 µg, ATGA0367-1 mg, ATGA0367-10, ATGA0367-20, ATGA0367-50, ATGA0367-100, ATGA0367-250, ATGA0367-500, ATGA0367-1
References & Citations
Miyake S., et al. (1987) Nucleic Acids Res. 14 (22) : 8805-8817.; ; Ohno S., et al. (1986) Nucleic Acids Res. 14 (13) : 5559.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_001740
Uniprot Accession Number
P04632
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPNS1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A431 cell lysate; ; Lane 3.: 293T cell lysate;
IF Description
ICC/IF analysis of CAPNS1 in HeLa cells. The cell was stained with ATGA0367 (1:100) . The secondary antibody (green) was used Alexa Fluor 488.

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