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Human Cyclin H antibody

CAT: 0112-ATGA0363-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0363-050Size:50 µL
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Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK) . This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.
Product Name Alternative
CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Cyclin H (1-323aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT3G6
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Cyclin H, CCNH, CAK, p34, p37, 6330408H09Rik, AI661354, AV102684, AW538719, CDK activating kinase, Cyclin dependent kinase activating kinase, Cyclin H, CyclinH, MO15 associated protein, P36, Cyclin-H, ATGA0363-10 µg, ATGA0363-20 µg, ATGA0363-50 µg, ATGA0363-100 µg, ATGA0363-250 µg, ATGA0363-500 µg, ATGA0363-1 mg, ATGA0363-10, ATGA0363-20, ATGA0363-50, ATGA0363-100, ATGA0363-250, ATGA0363-500, ATGA0363-1
References & Citations
Jeang KT., et al. (1998) J Biomed Sci. 5 (1) : 24-27.; ; Yankulov K., et al. (1998) Current Biology. 8 (13) : 447-449.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001230
Uniprot Accession Number
P51946
WB Description
The recombinant proteins (25ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Human Cyclin H. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate ; ; Lane 2.: Jurkat cell lysate ; ; Lane 3.: Ramos cell lysate ; ; Lane 4.: Balb-3T3 cell lysate {ATGA0363-WB2.jpg}

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