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Human GOT2 antibody

CAT: 0112-ATGA0357-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0357-050Size:50 µL
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Description
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. Also, GOT2 is a major participant in the malate-aspartate shuttle, which is a passage from the cytosol to the mitochondria. The two enzymes are homodimeric and show close homology. GOT2 has been seen to have a role in cell proliferation, especially in terms of tumor growth.
Product Name Alternative
Aspartate aminotransferase 2 mitochondrial, Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human GOT2 (30-430aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
AT8B11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
GOT2, ATGA0357-10 µg, ATGA0357-20 µg, ATGA0357-50 µg, ATGA0357-100 µg, ATGA0357-250 µg, ATGA0357-500 µg, ATGA0357-1 mg, ATGA0357-10, ATGA0357-20, ATGA0357-50, ATGA0357-100, ATGA0357-250, ATGA0357-500, ATGA0357-1
References & Citations
Yang H., et al. (2015) The EMBO journal. 34 (8) : 1110-1125.; ; Guidetti P., et al. (2007) Journal of neurochemistry. 102 (1) : 103-111.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_002071
Uniprot Accession Number
P00505
WB Description
The recombinant human GOT2 (50ng), cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: GOT2 Recombinant protein; ; Lane 2.: Ramos cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Balb/3T3 cell lysate {ATGA0357-WB2.jpg} The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Human GOT1; ; Lane 2.: Recombinant Human GOT2 {ATGA0357-WB3.jpg}
FACS Description
Flow cytometry analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of GOT2 in HeLa cells. The cell was stained with ATGA0357 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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