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Human DNAL1 antibody

CAT: 0112-ATGA0287-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0287-100Size:100 µL
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Description
DNAL1, also known as dynein light chain 1 axonemal, is component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. DNAL1 is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Homozygous mutations in the DNAL1 gene are associated with primary ciliary dyskinesia 16 (CILD16) which is a disorder characterized by abnormalities of motile cilia.
Product Name Alternative
Dynein light chain 1 axonemal, Dynein light chain 1, axonemal, C14orf168, CILD16
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human DNAL1 (1-190aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT29E4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
DNAL1, ATGA0287-10 µg, ATGA0287-20 µg, ATGA0287-50 µg, ATGA0287-100 µg, ATGA0287-250 µg, ATGA0287-500 µg, ATGA0287-1 mg, ATGA0287-10, ATGA0287-20, ATGA0287-50, ATGA0287-100, ATGA0287-250, ATGA0287-500, ATGA0287-1
References & Citations
Mazor. M., et al. (2011) Am J Hum Genet 88: 599-607.; ; Horvath. J., et al. (2005) Am J Respir Cell Mol Biol 33: 41-47.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_113615
Uniprot Accession Number
Q4LDG9
WB Description
The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Description
Flow cytometry analysis of DNAL1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of DNAL1 in HeLa cells. The cell was stained with ATGA0287 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of DNAL1 in A549 cells. The cell was stained with ATGA0287 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0287-IF2.jpg}

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