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Human SSR2 antibody

CAT: 0112-ATGA0277-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0277-050Size:50 µL
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Description
SSR2 belongs to the TRAP-beta family. SSR is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. The SSR consists of 2 subunits, a 34kD glycoprotein (alpha-SSR or SSR1) and a 22kD glycoprotein (beta-SSR or SSR2) .
Product Name Alternative
Tanslocon-associated protein subunit beta precursor, Tanslocon-associated protein subunit beta precursor, HSD25, TLAP, TRAP-BETA, TRAPB
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SSR2 (18-149aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT31G6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SSR2, ATGA0277-10 µg, ATGA0277-20 µg, ATGA0277-50 µg, ATGA0277-100 µg, ATGA0277-250 µg, ATGA0277-500 µg, ATGA0277-1 mg, ATGA0277-10, ATGA0277-20, ATGA0277-50, ATGA0277-100, ATGA0277-250, ATGA0277-500, ATGA0277-1
References & Citations
Chinen, K., et al. (1995) Cytogenet Cell Genet 70 (3-4) : 215-7.; ; Sikorska, M., et al. (2008) J Neurosci Res 86 (8) : 1680-93.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003136
Uniprot Accession Number
P43308
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSR2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: Jurkat cell lysate
IF Description
ICC/IF analysis of SSR2 in HeLa cells. The cell was stained with ATGA0277 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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