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Human MRPS25 antibody

CAT: 0112-ATGA0258-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0258-100Size:100 µL
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Description
MRPS25 is a 28S subunit protein that belongs to the ribosomal protein S5P family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. A pseudogene corresponding to this gene is found on chromosome 4
Product Name Alternative
28S ribosomal protein S25 mitochondrial, 28S ribosomal protein S25, mitochondrial, mitochondrial ribosomal protein S25, MRP-S25, RPMS25
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MRPS25 (1-173aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT38E7
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
MRPS25, 28S ribosomal protein S25 mitochondrial, 28S ribosomal protein S25, mitochondrial, mitochondrial ribosomal protein S25, MRP-S25, RPMS25, ATGA0258-10 µg, ATGA0258-20 µg, ATGA0258-50 µg, ATGA0258-100 µg, ATGA0258-250 µg, ATGA0258-500 µg, ATGA0258-1 mg, ATGA0258-10, ATGA0258-20, ATGA0258-50, ATGA0258-100, ATGA0258-250, ATGA0258-500, ATGA0258-1
References & Citations
Koc EC, Burkhart W, Blackburn K, et al. (2000) . J. Biol. Chem. 275 (42) : 32585-91.; ; Zhang Z, Gerstein M (2003) . Genomics 81 (5) : 468-80.; ; Cavdar Koc E, et al. (May 2001) . J Biol Chem 276 (22) : 19363-74.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_071942
Uniprot Accession Number
P82663
WB Description
The cell lysates of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MRPS25 antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: anti-human MRPS25 antibody 1:500; ; Lane 2.: anti-human MRPS25 antibody 1:1000; ; Lane 3.: anti-human MRPS25 antibody 1:5000

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