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Human KCTD15 antibody

CAT: 0112-ATGA0178-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0178-100Size:100 µL
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Description
KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.
Product Name Alternative
BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628, potassium channel tetramerisation domain containing 15
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human KCTD15 (1-234) purified from E.coli
Clonality
Monoclonal
Isotype
IgG3 κ
Clone
AT4C3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
KCTD15, ATGA0178-10 µg, ATGA0178-20 µg, ATGA0178-50 µg, ATGA0178-100 µg, ATGA0178-250 µg, ATGA0178-500 µg, ATGA0178-1 mg, ATGA0178-10, ATGA0178-20, ATGA0178-50, ATGA0178-100, ATGA0178-250, ATGA0178-500, ATGA0178-1
References & Citations
Willer CJ, et al. (2010) Nat. Genet, 41 (1) :24-34.; ; Elks CE, et al. (2010) PLoS Med, 7 (5) :e1000284.; ; Strausberg RL, et al. (2002) Proc. Natl. Acad. Sci. U.S.A, 99 (26) :16899-903.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_076981
Uniprot Accession Number
Q96SI1
WB Description
The cell lysate of HeLa (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KCTD15 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : 293T cell lysate; ; Lane 2 : KCTD15 Transfected 293T cell lysate {ATGA0178-WB2.jpg}
IF Description
Immunofluorescence of human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .

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