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Human MYL2 antibody

CAT: 0112-ATGA0151-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0151-050Size:50 µL
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Description
Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy
Product Name Alternative
Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MYL2 (1-166aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT3B2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
MYL2, ATGA0151-10 µg, ATGA0151-20 µg, ATGA0151-50 µg, ATGA0151-100 µg, ATGA0151-250 µg, ATGA0151-500 µg, ATGA0151-1 mg, ATGA0151-10, ATGA0151-20, ATGA0151-50, ATGA0151-100, ATGA0151-250, ATGA0151-500, ATGA0151-1
References & Citations
Macera MJ, et al., (1992) Genomics. 13 (3) :829-31.; ; Poetter K, et al., (1996) Nat Genet. 13 (1) :63-9.
Other References
Beveridge RD, et al. The leukemia-associated Rho guanine nucleotide exchange factor LARG is required for efficient replication stress signaling. (Cell Cycle. 2014) {https://pubmed.ncbi.nlm.nih.gov/25485589/}; ; Russo V, et al. Porous, Ventricular Extracellular Matrix-Derived Foams as a Platform for Cardiac Cell Culture. (Biores Open Access. 2015) {https://pubmed.ncbi.nlm.nih.gov/26487982/}; ; Mourino-Alvarez L, et al. A comprehensive study of calcific aortic stenosis: from rabbit to human samples. (Dis Model Mech. 2018) {https://pubmed.ncbi.nlm.nih.gov/29752279/}; ; Acharya BR, et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. (Dev Cell. 2018) {https://pubmed.ncbi.nlm.nih.gov/30318244/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_000423
Uniprot Accession Number
P10916
WB Description
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse heart tissue lysate
FACS Description
Flow cytometry analysis of MYL2 in A431 cells. The cell was stained with ATGA0151 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of MYL2 in A431 cells. The cell was stained with ATGA0151 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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