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Human IL-6 antibody

CAT: 0112-ATGA0123-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0123-050Size:50 µL
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Description
Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length that plays critical roles in host defense, immune response and hematopoiesis. IL-6 produced by T cells, macrophages, fibroblasts, endothelial and other cells. IL-6 induces proliferation or differentiation in many cell types including B cells, thymocytes and T cells. IL-6, in concert with TGF-Beta, is important for developing Th17 responses. The IL-6 receptor is a trimeric complex composed of an IL-6-specific a chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.
Product Name Alternative
Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant IL-6 (30-212aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT1H6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGA0123-10 µg, ATGA0123-20 µg, ATGA0123-50 µg, ATGA0123-100 µg, ATGA0123-250 µg, ATGA0123-500 µg, ATGA0123-1 mg, ATGA0123-010, ATGA0123-020, ATGA0123-050, ATGA0123-100, ATGA0123-250, ATGA0123-500, ATGA0123-01M
References & Citations
Heinrich P.C., et al. (1998) Biochem J. 334: 297-314.; ; Heinrich P.C., et al. (1998) Z Ernahrungswiss. 37: 43-49.; ; Jones S.A. (2005) J Immunol. 175: 3463-3468.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_000591
Uniprot Accession Number
P05231
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-6 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: Raw264.7 cell lysate ; ; Lane 4.: PC3 cell lysate ; ; Lane 5.: THP-1 cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: MCF7 cell lysate ; ; Lane 8.: K562 cell lysate
FACS Description
Flow cytometry analysis of IL-6 in LNCaP cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .Flow cytometry analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{2004010104258138.jpg}
IF Description
ICC/IF analysis of IL-6 in A549 cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of IL-6 in HeLa cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010104255381.jpg}ICC/IF analysis of IL-6 in Hep3B cells. The cell was stained with ATGA0123 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010104258221.jpg} {ATGA0123-IF3b.jpg}

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