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Human PD-1 antibody

CAT: 0112-APD0811-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-APD0811-050Size:50 µL
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Description
PD-1 is an immunoreceptor that belongs to the immunoglobulin (Ig) superfamily, also known as Programmed Cell Death 1. It is expressed on activated T cell, B cells, myeloid cells and a subset of thymocytes. PD-1 interacts with expressed PDL1 and PDL2 to inhibit the proliferation and activation of T cells and B cells. Both PDL1 and PDL2 are expressed in a wide variety of normal tissues including heart, lung, placenta, spleen and kidney. PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases.
Product Name Alternative
Programmed cell death 1, CD279, PD1, hPD-1, hPD-l, PDCD1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PDCD1 (21-167aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
4F12
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PD1, APD0811-10 µg, APD0811-20 µg, APD0811-50 µg, APD0811-100 µg, APD0811-250 µg, APD0811-500 µg, APD0811-1 mg, APD0811-10, APD0811-20, APD0811-50, APD0811-100, APD0811-250, APD0811-500, APD0811-1
References & Citations
Freeman GJ, et al., (2000) J Exp Med. 192 (7) :1027-34.; ; Latchman Y, et al., (2001) Nat Immunol. 2 (3) :261-8.; ; Nishimura H, HonjoT., (2001) Trends Immunol. 22 (5) :265-8.; ; Okazaki T, et al., (2001) Proc Natl Acad Sci USA. 98 (24) :13866-71.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
AAH74740
Uniprot Accession Number
Q15116
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PD-1 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Raji cell lysate
FACS Description
Flow cytometry analysis of PD-1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of PD-1 in HeLa cells. The cell was stained with APD0811 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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