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Human Hexokinase antibody

CAT: 0112-AHK0715-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-AHK0715-100Size:100 µL
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Description
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.
Product Name Alternative
BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase PI, Hexokinase type I, Hexokinase-A, HK I, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HXK1, mHk1-s, tumor isozyme, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase 4, HK1, HK2, HK3, HK4
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Hexokinase1 (1-917aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
4D7
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
BB404130, Brain form hexokinase, dea, EC 2.7.1.1, Glycolytic enzyme, HEXOKIN, Hexokinase, Hexokinase 1, Hexokinase 2, Hexokinase 3, Hexokinase PI, Hexokinase type I, Hexokinase type II, Hexokinase type III, Hexokinase1, Hexokinase2, Hexokinase3, Hexokinase-1, Hexokinase-2, Hexokinase-3, Hexokinase-A, HK I, HK II, HK III, HK1, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HK2, HK3, HKI, HKII, HKIII, HXK1, HXK2, HXK3, HXK 1, HXK 2, HXK 3, HXK-1, HXK-2, HXK-3, mHk1-s, Muscle form hexokinase, Tumor isozyme, AHK0715-10 µg, AHK0715-20 µg, AHK0715-50 µg, AHK0715-100 µg, AHK0715-250 µg, AHK0715-500 µg, AHK0715-1 mg, AHK0715-010, AHK0715-020, AHK0715-050, AHK0715-100, AHK0715-250, AHK0715-500, AHK0715-01M
References & Citations
Ellison W R. et al., (1975) J Biol Chem 250:1864-1871.; ; Furuta H. et al., (1996) Genomics 36 (1) :206-9.; ; Jon E. et al., (2003) J.Exp Biology 206:2049-2057.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000179
Uniprot Accession Number
P19367
WB Description
The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Hexokinase 1 recombinant protein; ; Lane 2.: Hexokinase 2 recombinant protein; ; Lane 3.: Hexokinase 3 recombinant protein; ; Lane 4.: Hexokinase 4 recombinant protein; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: K562 cell lysate; ; {AHK0715-WB2.jpg}The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain Tissue lysate {AHK0715-WB3.jpg}
IF Description
ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained with AHK0715 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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