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Human Dapper 3/DACT3 antibody

CAT: 0112-ADA0925-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ADA0925-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.
Product Name Alternative
Thymus expressed gene 3-like, Dapper antagonist of beta-catenin homolog 3 (Xenopus laevis), Arginine rich region 1, MGC15476
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human DACT3 (89-202aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
2A5
Conjugation
Unconjugated
Applications
ELISA, WB, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
DACT3, ADA0925-10 µg, ADA0925-20 µg, ADA0925-50 µg, ADA0925-100 µg, ADA0925-250 µg, ADA0925-500 µg, ADA0925-1 mg, ADA0925-10, ADA0925-20, ADA0925-50, ADA0925-100, ADA0925-250, ADA0925-500, ADA0925-1
References & Citations
Jiang X, et al., (2008) Cancer Cell. 13 (6) :529-41.; ; Fisher DA, et al., (2006) Dev Dyn. 235 (9) :2620-30.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Development
NCBI Accession Number
NP_659493
Uniprot Accession Number
Q96B18
WB Description
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DACT3 antibody (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse Brain tissue lysate
IHC description
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

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