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Human Integrin beta 1/CD29 antibody

CAT: 0112-ACD0823-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ACD0823-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD29, also known as Integrin beta 1, is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. Interaction between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Product Name Alternative
Integrin beta 1, Integrin beta 1, CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, Fibronectin receptor subunit beta, Glycoprotein Iia, GPIIA, VLA-4 subunit bet
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CD29 (34-141aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
K2D5
Conjugation
Unconjugated
Applications
ELISA, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VLA-BETA, VLAB, VLA-4 subunit beta, NRK2, Nicotinamide riboside kinase 2, MSK12, MIBP, MDF2, ITGB1, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta-1 isoform 1A, Integrin beta 1, Integrin b1, Il-6, IL6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ACD0823-10 µg, ACD0823-20 µg, ACD0823-50 µg, ACD0823-100 µg, ACD0823-250 µg, ACD0823-500 µg, ACD0823-1 mg, ACD0823-010, ACD0823-020, ACD0823-050, ACD0823-100, ACD0823-250, ACD0823-500, ACD0823-01M
References & Citations
He L, et al., (2003) Blood 102 (10) :3652-3657.; ; Arrequi C, et al., (2000) J cell Biol 149 (6) :1263-1274.; ; Martin-padura I, et al., (1994) J Biol Chem 269:6124-6132.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_002202
Uniprot Accession Number
P05556
FACS Description
Flow cytometry analysis of CD29 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IF Description
ICC/IF analysis of CD29 in HepG2 cells. The cell was stained with ACD0823 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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