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Human BHMT antibody

CAT: 0112-ABH0708-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ABH0708-100Size:100 µL
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Description
Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.
Product Name Alternative
Betaine-homocysteine S-methyltransferase 1, BHMT1, Betaine homocysteine methyltransferase
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BHMT (1-406aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3D6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Betaine homocysteine methyltransferase, Betaine homocysteine S methyltransferase, Betaine homocysteine S methyltransferase 1, Betaine-homocysteine methyltransferase, Betaine-homocysteine methyltransferase Betaine homocysteine methyltransferase, Betaine-homocysteine S-methyltransferase 1, BHMT, BHMT (Betaine-homocysteine methyltransferase), BHMT1, EC 2.1.1.5, ABH0708-10 µg, ABH0708-20 µg, ABH0708-50 µg, ABH0708-100 µg, ABH0708-250 µg, ABH0708-500 µg, ABH0708-1 mg, ABH0708-010, ABH0708-020, ABH0708-050, ABH0708-100, ABH0708-250, ABH0708-500, ABH0708-01M
References & Citations
Mckeever MP et al., (1991) Clin Sci 81 (4) :551-556; ; Norihiko F et al., (2001) Biochem J 129:313-320; ; Francisco G et al., (2005) Biochem J 391:589-599; ; Catherine M et al., (2005) Biochem J 392:443-448; ; Janet D. S et al., (2006) Biochem J 395:363-371
Other References
Li S, et al. Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha. (Am J Physiol Endocrinol Metab. 2009) {https://pubmed.ncbi.nlm.nih.gov/19158324/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH12616
Uniprot Accession Number
Q93088
WB Description
The extracts of mouse liver (each 20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
FACS Description
Flow cytometry analysis of BHMT in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
IF Description
ICC/IF analysis of BHMT in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BHMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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