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UBE3B Polyclonal Antibody

CAT: 1027-RA35814-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:1027-RA35814-01Size:50 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Ubiquitin protein ligase E3B (UBE3B) Homo sapiens The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [pro
Product Name Alternative
UBE3B; Ubiquitin-protein ligase E3B
Gene ID
89910
Swiss Prot
Q7Z3V4
Cross Reactivity
Human; Rat; Mouse
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC-p; IF (paraffin section) ; ELISA
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Molecular Weight
123
Storage Conditions
-20°C/1 year
Protein Weight
123

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