M-RIP Polyclonal Antibody

• Background:

  Function:Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells., similarity:Contains 1 PH domain., similarity:Contains 2 PH domains., subcellular location:Colocalizes with F-actin., subunit:Binds F-actin through its N-terminus (By similarity) . Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.

• Product Name Alternative:

  MPRIP; KIAA0864; MRIP; RHOIP3; Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip

• Gene ID:

  23164

• Swiss Prot:

  Q6WCQ1

• Cross Reactivity:

  Human; Mouse; Rat

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  IHC-p; IF (paraffin section) ; WB; ELISA

• Dilution:

  WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Buffer:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Molecular Weight:

  110

• Storage Conditions:

  -20°C/1 year

• Protein Weight:

  110