Recombinant Human GM-CSF (E. coli)

• Product Name Alternative:

  Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF

• Accession Number:

  P04141

• Endotoxin:

  < 0.01 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

• Bioactivity:

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 4-20 pg/ml. (QC verified)

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  Mol Mass:14.6 KDa; APMol Mass:13-17 KDa, reducing conditions

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.