Recombinant Human EGF

• Product Name Alternative :

  Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

• Accession Number :

  P01133

• Endotoxin :

  < 0.01 EU/µg as determined by LAL test.

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

• Bioactivity :

  Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml. (QC verified)

• Reconstitution :

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight :

  Mol Mass:6.2 KDa; APMol Mass:11 KDa, reducing conditions

• Shipping Conditions :

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

• Formulation :

  Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.