Leptin qA Ovine

• Description:

  Leptin Antagonist Quadruple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A/I42A mutant.

• Source:

  Escherichia coli.

• Buffer:

  The protein was lyophilized from a concentrated (1 mg/mL) solution with 045mM NaHCO3.

• Storage Conditions:

  Lyophilized Leptin-Antagonist Quadruple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 LEP-qA mutant mg/mL and up to 2mg/mL and filter sterilization Leptin mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested.

• Sequence Similarities:

  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.