CAS9 S. Pyogenes

• Description:

  Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.

• Swiss Prot:

  Q99ZW2

• Source:

  Escherichia Coli.

• Buffer:

  The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.

• Storage Conditions:

  Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.