MCherry Antibody

• Background:

  MCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004) . Fluorescent proteins are commonly used to tag proteins in the study of their expression and in transgenic research. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.

• Synonyms:

  DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

• Host:

  Mouse

• Cross Reactivity:

  Tags, Others

• Target:

  MCherry

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  1C51

• Conjugation:

  Unconjugated

• Source:

  Recombinant full length protein

• Applications:

  WB, IHC

• Purification:

  Purified by Protein G.

• Concentration:

  Lot Dependent

• Dilution:

  WB (1:300-5000), IHC ()

• Buffer:

  PBS + 50% glycerol and 5 mM NaN3

• Modification:

  Unmodified

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• Specificity:

  Specific for the ~28 kDa mCherry protein