MAP2K1/2 Recombinant Antibody, PE Conjugated

• Background:

  A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

• Synonyms:

  Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1; MAP2K1; MEK1; MKK2; PRKMK1; MAPKK1; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2; MAP2K2; MEK2; MKK2; PRKMK2; MAPKK2

• Gene ID:

  5604; 5605

• Swiss Prot:

  Q02750; P36507

• Cellular Locus:

  Cytoplasm, Nucleus, Mitochondrion

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  MAP2K1/2

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  4H7

• Conjugation:

  PE

• Source:

  Human MAP2K1 + MAP2K2 aa 1 to the C-terminus

• Applications:

  WB, IF

• Purification:

  Purified by Protein A.

• Excitation Emission:

  496,564nm/578nm

• Concentration:

  Lot dependent

• Dilution:

  WB (1:300-5000), IF ()

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Specificity:

  The antibody will detect both MAP2K1 and MAP2K2

• Gene ID URL:

  5604; 5605

• Cross Reactivity Details:

  Crucian carp (Carassius carassius)