FEN1 Polyclonal Antibody

• Background :

  Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

• Synonyms :

  DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specic endonuclease 1; Flap structure-specic endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2.

• Gene ID :

  2237

• Host :

  Rabbit

• Cross Reactivity :

  Mouse

• Immunogen :

  165-260/380

• Target :

  FEN1

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Conjugation :

  Unconjugated

• Source :

  KLH conjugated synthetic peptide derived from human FEN1

• Applications :

  WB, IHC-P, IF (IHC-P)

• Purification :

  Purified by Protein A.

• Concentration :

  1µg/µl

• Dilution :

  WB (1:300-5000), IHC-P (1:200-400), IF (IHC-P) (1:50-200)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL :

  2237

• Predicted Cross Reactivity :

  Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit