RNF31 Polyclonal Antibody

• Background:

  E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.

• Synonyms:

  HOIP; ZIBRA; E3 ubiquitin-protein ligase RNF31; HOIL-1-interacting protein; RING finger protein 31; Zinc in-between-RING-finger ubiquitin-associated domain protein; RNF31

• Gene ID:

  55072

• Swiss Prot:

  Q96EP0

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Immunogen:

  671-780/1072

• Target:

  RNF31

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human RNF31

• Applications:

  IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  IHC-P (IHC-P=1:100-500), IHC-F (IHC-F=1:100-500), IF (IF=1:50-200), Flow-Cyt (Flow-Cyt=2ug/Test), ICC/IF (ICC/IF=1:50)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  55072