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Phospho-Cdc25B (Ser149) Polyclonal Antibody

CAT: 0498-bs-8445RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-8445RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity) .
Synonyms
AI604853; M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; Cdc25b; Cdc25m2
Gene ID
12531
Swiss Prot
P30306
Modification Site
Ser149
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-Cdc25B (Ser149)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from mouse Cdc25B around the phosphorylation site of Ser149
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
12531
Predicted Cross Reactivity
Human, Rat

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