RPS3 Polyclonal Antibody

• Background:

  Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699) . Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413) . Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971) . Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345) . Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840) . Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571) . Enhances the uracil excision activity of UNG1 (PubMed:18973764) . Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764) . When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537) . Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931) . Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535) . Represses its own translation by binding to its cognate mRNA (PubMed:20217897) . Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744) . Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551) . Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002) . Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787) . Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408) .

• Synonyms:

  S3; 40S ribosomal protein S3; RPS3; OK/SW-cl.26

• Gene ID:

  6188

• Swiss Prot:

  P23396

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse

• Immunogen:

  101-200/243

• Target:

  RPS3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human RPS3

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  6188

• Predicted Cross Reactivity:

  Rat, Zebrafish, Non-Human Primate, Xenopuslaevis