Plexin A1 (Sema Domain) Antibody

• Background:

  Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. This region in Plexin-A1 binds the RhoGTPases, Rnd1 and RhoD. Recruitment of Rnd1 has been implicated in the cytoskeletal collapse that occurs after semaphorin-mediated activation of Plexin-A1, while RhoD may block this collapsing activity through interaction with the cytoplasmic region of Plexin-A1. The expression of Plexin-A1, along with the co-receptor Neuropilin-1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.<

• Synonyms:

  PLXN1, NOV, Sema3A

• Swiss Prot:

  Q9UIW2

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  Plexin A1 (Sema Domain)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  Plexin-A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the semaphorin domain in human Plexin-A1.

• Applications:

  WB

• Purification:

  Antigen Affinity purification

• Dilution:

  WB (1:300-5000)

• Buffer:

  PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

• Modification:

  Unmodified

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• Specificity:

  The antibody detects a 220 kDa* protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of adult mouse brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1. This sequence is highly conserved in rat and mouse Plexin-A1, and has low homology to other Plexin family members.