Products for Research Use Only
RegisterLogin

Phosphotyrosine Antibody

CAT: 0498-bs-70609RSize: 100 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0498-bs-70609RSize:100 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
Synonyms
Phosphotyrosine mAb
Modification Site
P-Tyr
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat, Chicken
Target
Anti-Phosphotyrosine
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences.
Applications
WB, IP
Purification
Antigen Affinity purification
Dilution
WB (1:300-5000), IP (1-2ug)
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. All peptide sequences used are highly conserved phosphotyrosine motifs.

Related Products

CatalogName
bsm-33205MPhosphotyrosine Monoclonal Antibody
bs-10497PBPhosphotyrosine, BSA Conjugated
bsm-33205M-Cy3Phosphotyrosine Monoclonal Antibody, Cy3 Conjugated
bsm-33205M-APCPhosphotyrosine Monoclonal Antibody, APC Conjugated
bsm-33205M-FITCPhosphotyrosine Monoclonal Antibody, FITC Conjugated
bsm-33205M-BiotinPhosphotyrosine Monoclonal Antibody, Biotin Conjugated
bsm-33205M-PEPhosphotyrosine Monoclonal Antibody, PE Conjugated
bsm-33205M-Cy5Phosphotyrosine Monoclonal Antibody, Cy5 Conjugated
bsm-33205M-HRPPhosphotyrosine Monoclonal Antibody, HRP Conjugated
bsm-33205M-RBITCPhosphotyrosine Monoclonal Antibody, RBITC Conjugated

Alternative Products

CatalogName
GWB-9474D7Phosphotyrosine Antibody

Popular Products