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ENOS Antibody

CAT: 0498-bs-70569RSize: 100 µLDry Ice: NoHazardous: No
CAT#:0498-bs-70569RSize:100 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme) . Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation.
Synonyms
Endothelial Nitric Oxide Synthase, eNOS, ecNOS, NOS-III, NOS3, NOSIII
Swiss Prot
P70313
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
ENOS
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
This antibody was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region.
Applications
WB, IHC
Purification
Antigen Affinity purification
Dilution
WB (1:300-5000), IHC ()
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Unmodified
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family members.

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