Products for Research Use Only
RegisterLogin

EEF2K (Ser-359), Phosphospecific Antibody

CAT: 0498-bs-70469RSize: 100 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0498-bs-70469RSize:100 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. eEF2 kinase (eEF2K) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2K is normally dependent on Ca2+ ions and calmodulin, and can be activated by PKA in response to elevated cAMP levels during cell stress- or starvation-related conditions. Regulation of eEF2K occurs through phosphorylation at multiple sites. Ser-78 phosphorylation is required for calmodulin binding and eEF2K activity, while phosphorylation of Ser-500 is required for Ca2+/calmodulin-independent kinase activity. Thr-348 is an autophosphorylation site that is required for kinase activity. Inhibitory phosphorylation may also regulate eEF2K, since phosphorylation at Ser-359 by SAPK4/p38δ causes inactivation of eEF2K. Thus, multisite phospho-regulation of eEF2K may be important for proper control of eEF2K activity and protein translation.
Synonyms
EEF-2, eEF-2K, CaMK-III, eukaryotic elongation factor
Swiss Prot
O00418
Modification Site
Ser-359
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
EEF2K (Ser-359)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
Phospho-eEF2K (Ser-359) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-359 in human eEF2K.
Applications
WB
Purification
Antigen Affinity purification
Dilution
WB (1:300-5000)
Buffer
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Modification
Phosphorylation
Storage Conditions
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Specificity
The antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAGE immunoblots of human recombinant eEF2K autophosphorylated in the presence of Ca2+ and Calmodulin. This reactivity is removed after lambda phosphatase treatment. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.

Related Products

CatalogName
bs-70472REEF2K (Ser-500), Phosphospecific Antibody
bs-70471REEF2K (Ser-445), Phosphospecific Antibody
bs-70467REEF2K (Ser-78), Phosphospecific Antibody
bs-70470REEF2K (Ser-398), Phosphospecific Antibody
bs-70468REEF2K (Thr-348), Phosphospecific Antibody
bs-70442RCXCR4 (Ser-324/Ser-325), Phosphospecific Antibody
bs-70700RTRPM7 (Ser-1513), Phosphospecific Antibody
bs-70662RSHP1 (Ser-591), Phosphospecific Antibody
bs-70597RPaxillin (Ser-178), Phosphospecific Antibody
bs-70507RGirdin (Ser-1674), Phosphospecific Antibody

Alternative Products

CatalogName
EP4431Anti-eEF2K (Ser-359), Phosphospecific Antibody
EP5461Anti-eEF2K (Ser-445), Phosphospecific Antibody
EP4451Anti-eEF2K (Ser-500), Phosphospecific Antibody
EP5441Anti-eEF2K (Ser-398), Phosphospecific Antibody
EP4391Anti-eEF2K (Ser-78), Phosphospecific Antibody
MBS474346-01eEF2K (Ser-359), phospho-specific Antibody
E11-8340BEEF2K (Ab-359) Antibody
EP4411Anti-eEF2K (Thr-348), Phosphospecific Antibody
CP4251Anti-CXCR4 (Ser-324/Ser-325), Phosphospecific Antibody
TP5671Anti-TRPM7 (Ser-1513), Phosphospecific Antibody

Popular Products