TREM2 Antibody

• Background:

  Triggering receptor expressed on myeloid cells 2, TREM2, is a type I transmembrane innate immune receptor predominantly expressed by microglia in the central nervous system and is genetically linked to the risk for Alzheimer’s disease (Schmid, C.D., et al., 2002) . TREM2 has been shown to be a specific marker for alternatively activated microglia known as M2 cells (Cherry, J.D. et al., 2014) . TREM2 binds to its adaptor, DNAX-activating protein (DAP12) on the surface of microglia to enact innate immune responses and downstream cellular responses or signaling pathways (Jay, T.R, et al., 2017) . TREM2 has important roles in microglial phagocytosis of apoptotic neurons, damaged myelin, and amyloid plaques (Gratuze, M., et al., 2018) . In early brain development, TREM2 is essential for synaptic pruning (Filipello, F. et al., 2018) .

• Synonyms:

  Triggering receptor expressed on myeloid cells 2

• Gene ID:

  54209

• Swiss Prot:

  Q9NZC2

• Host:

  Rabbit

• Cross Reactivity:

  Mouse

• Target:

  TREM2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  Recombinant human TREM2 protein expressed in and purified from E. Coli.

• Applications:

  WB

• Purification:

  Antigen Affinity purification from Pooled whole antiserum

• Concentration:

  Lot Dependent

• Dilution:

  WB (1:300-5000)

• Buffer:

  10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• Specificity:

  Specific for endogenous levels of the ~28 kDa TREM2 protein.

• Gene ID URL:

  54209

• Predicted Cross Reactivity:

  Human