SOX2 Antibody

• Background:

  SOX2 (SRY (sex-determining region Y) -box 2) is one of 20 members of the Sox family proteins that play essential roles in cell differentiation, development, and organogenesis. SOX2, a group B Sox family protein, is one of the master transcriptional factors, along with Oct3/4 (Nichols, J., et al, 1998) and PAK6 (Kamachi, Y. et al, 2001) that are important in maintaining the pluripotency of embryonic stem cells, thus making SOX2 an excellent marker of neural stem cells. SOX2 has also been identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinomas due to peak amplification on chromosome 3q26.33 which contains the SOX2 gene transcription factor (Bass, A.J., et al, 2009) . Additionally, Sox2 has been reported to be highly deregulated in glioblastomas (Gangemi, R. M. R, et al, 2009) and breast cancer (Rodriguez-Pinilla, S.M., et al, 2007) .

• Synonyms:

  SRY box 2, sex-determining region Y box 2

• Gene ID:

  6657

• Swiss Prot:

  P48431

• Host:

  Rabbit

• Cross Reactivity:

  Mouse

• Target:

  SOX2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  Recombinant protein from the C-terminal region of human SOX2.

• Applications:

  WB

• Purification:

  Antigen Affinity purification from Pooled whole antiserum

• Concentration:

  Lot Dependent

• Dilution:

  WB (1:300-5000)

• Buffer:

  10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

• Specificity:

  Specific for endogenous levels of the ~35 kDa SOX2 protein.

• Gene ID URL:

  6657

• Predicted Cross Reactivity:

  Human