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MYF5 Polyclonal Antibody

CAT: 0498-bs-6936R-01Size: 20 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-6936R-01Size:20 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin) . Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.
Synonyms
Myf-5; bHLHc2; Class C basic helix loop helix protein 2; Class C basic helix-loop-helix protein 2; Myf 5; Myf-5; Myf5; MYF5_HUMAN; Myogenic factor 5.
Gene ID
4617
Swiss Prot
P13349
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Mouse, Rat
Immunogen
61-160/255
Target
MYF5
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human MYF5
Applications
ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
4617
Predicted Cross Reactivity
Human, Cow, Sheep, Pig, Horse, Chicken, Rabbit

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