FbpB Polyclonal Antibody

• Background:

  The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs) . They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha, alpha-trehalose dimycolate (TDM, cord factor) . They catalyze the transfer of a mycoloyl residue from one molecule of alpha, alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM

• Synonyms:

  M.tuberculosis; Antigen 85 complex B;30 kDa extracellular protein; Mycobacterium; Mycolyl transferase 85B; A85B_MYCTU; Antigen 85-B; 30 kDa extracellular protein; 85B; Ag85B; Extracellular alpha-antigen; Fibronectin-binding protein B.

• Gene ID:

  885785, 886132

• Swiss Prot:

  P9WQP1, P9WQP3

• Cellular Locus:

  Secreted

• Host:

  Rabbit

• Cross Reactivity:

  Bacteria

• Immunogen:

  115-154/325

• Target:

  FbpB

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Antigen 85 complex B

• Applications:

  ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Specificity:

  This antibody will detect both Mycobacterium tuberculosis Ag85A and Mycobacterium tuberculosis Ag85B

• Gene ID URL:

  885785, 886132

• Cross Reactivity Details:

  Mycobacteriumtuberculosis