Phospho-RAF1 (Ser339) Polyclonal Antibody, APC Conjugated

• Background:

  Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) . The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1) . Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

• Synonyms:

  NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; RAF1; RAF

• Gene ID:

  5894

• Swiss Prot:

  P04049

• Modification Site:

  Ser339

• Cellular Locus:

  Cytoplasm, Nucleus, Cell membrane

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse

• Target:

  Phospho-RAF1 (Ser339)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  APC

• Source:

  KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Ser339

• Applications:

  WB, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Excitation Emission:

  650nm/660nm

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Gene ID URL:

  5894

• Predicted Cross Reactivity:

  Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit