Phospho-Caspase-9 (Ser196) Polyclonal Antibody

• Background:

  Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) . Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

• Synonyms:

  MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6; CASP9

• Gene ID:

  842

• Swiss Prot:

  P55211

• Modification Site:

  Ser196

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  Phospho-Caspase-9 (Ser196)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic phosphopeptide derived from human CASP9 around the phosphorylation site of Ser196

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  842