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Phospho-DDIT3 (Ser30) Polyclonal Antibody

CAT: 0498-bs-5177RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-5177RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Synonyms
Growth arrest and DNA damage-inducible 153; C/EBP homologous protein; C/EBP Homology Protein; CEBPZ; CHOP 10; CHOP; CHOP10; DDIT 3; DDIT3; DNA Damage Inducible Transcript 3; GADD 153; Growth Arrest and DNA Damage Inducible Protein 153; Growth arrest and DNA damage inducible protein GADD153; MGC4154; DDIT3_HUMAN.
Gene ID
1649
Modification Site
Ser30
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Rat
Target
Phospho-DDIT3 (Ser30)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human GADD153 around the phosphorylation site of Ser30
Applications
ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
1649
Predicted Cross Reactivity
Mouse, Dog, Cow, Sheep, Pig, Rabbit

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