DNMT3A Polyclonal Antibody

• Background:

  Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

• Synonyms:

  DNMT3A2, TBRS

• Gene ID:

  1788

• Swiss Prot:

  Q9Y6K1

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Target:

  DNMT3A

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Source:

  Polyclonal antibody raised in rabbit against human DNMT3A, (DNA methyltransferase 3A), using a KLH-conjugated synthetic peptide corresponding to amino acids 44-58

• Applications:

  WB, ELISA, IP

• Purification:

  Purified by Protein A.

• Concentration:

  0.75µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IP (1-2ug)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C for 12 months.

• Gene ID URL:

  1788