EHMT1 Polyclonal Antibody

• Background :

  Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me) . Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

• Synonyms :

  Histone-lysine N-methyltransferase EHMT1, EHMT1, GLP1, Lysine N-methyltransferase 1D, H3-K9-HMTase 5

• Gene ID :

  79813

• Swiss Prot :

  Q9H9B1

• Host :

  Rabbit

• Cross Reactivity :

  Human, Mouse

• Target :

  EHMT1

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Source :

  Polyclonal antibody raised in rabbit against mouse Ehmt1, using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein

• Applications :

  WB, ELISA

• Purification :

  Whole antiserum from rabbit containing 0.05% azide

• Concentration :

  Not Determined

• Dilution :

  WB (1:300-5000), ELISA (1:500-1000)

• Buffer :

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification :

  Unmodified

• Storage Conditions :

  Store at -20°C for 12 months.

• Gene ID URL :

  79813