Phospho-PRKAR2A (Ser99) Polyclonal Antibody, HRP Conjugated

• Background:

  Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

• Synonyms:

  PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A

• Gene ID:

  5576

• Swiss Prot:

  P13861

• Modification Site:

  Ser96

• Cellular Locus:

  Cytoplasm, Cell membrane

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Target:

  Phospho-PRKAR2A (Ser99)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  HRP

• Source:

  KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96

• Applications:

  WB, ELISA, IHC-P, IHC-F

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Specificity:

  This phosphorylation site is homologous to that of Ser97 in Rat.

• Gene ID URL:

  5576

• Predicted Cross Reactivity:

  Mouse, Rat