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PKA gamma Polyclonal Antibody

CAT: 0498-bs-3964RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-3964RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PKA (or cAPK) is a cyclic AMP dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, plus gene transcription. PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic) . Two families of R subunit (RI and RII) and three C subunit isoforms (C alpha, C beta, and C gamma) have been identified each possessing distinct cAMP binding properties and resulting in different phosphorylation states. C subunit is activated through autophosphorylation and direct phosphorylation at Thr197 by PDK-1. Tissue specific expression of C gamma, indicates pressure on C gamma during evolution, acting to modulate it in a functionally specific way. Certain amino acid substitutions make C gamma a distinct member of the cAMP dependent subfamily of protein kinases, and suggest that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits.
Synonyms
KAPG; PKA C gamma; PRKACG; Protein kinase cAMP dependent catalytic gamma; Serine threonine protein kinase; KAPCG_HUMAN.
Gene ID
5568
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Immunogen
251-351/351
Target
PKA gamma
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic peptide derived from human PKA gamma
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
5568
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken

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